Workshop: Workshop on Fundamentals of Mass Spectrometry-based Proteomics for Beginners        

Date: Apr 28-30, 2021

Timings: 9:00 AM-6:30 PM

Venue: Center for Systems Biology and Molecular Medicine, Yenepoya Research Centre, Yenepoya (Deemed to be University)


Schedule


Workshop Program

Day 1:

Tutorial Lectures

  • Introduction to proteomics
  • Fractionation strategies
  • Sample preparation strategies
    		•

    Day 2:

    Tutorial Lectures

  • Basics of LC-MS/MS analysis
  • LC-MS/MS operation
  • Key concepts of Mass spectrometry data analysis
    		•

    Day 3:

    Tutorial Lectures

  • Quantitative proteomics
  • Validation of proteomics data
  • Biological interpretation of proteomics data
  • TMT labeling

    • Hands on training

  • Sample preparation I (In-gel and in-solution digestion)
    		•

    Hands on training

  • Sample preparation II (peptide extraction, C18 clean-up)
  • Data analysis for qualitative proteomics
    		•

    Hands on training

  • Data analysis for quantitative proteomics